Research & Educational Use Only. This protocol guide is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional before beginning any research protocol.
Myostatin Inhibitor
Weekly Protocol Guide
Follistatin 344 Protocol Guide
Follistatin 344 is an activin-binding protein that functions as a myostatin inhibitor. By binding and neutralizing myostatin (a negative regulator of muscle growth), it is studied for its potential role in muscle hypertrophy and tissue remodeling. Administered subcutaneously every other day.
Protocol Overview
- Compound
- Follistatin 344 (Follistatin 344 (FST-344))
- Category
- Myostatin Inhibitor
- Vial Size
- 1mg (1,000mcg)
- Reconstitution
- 1mL bacteriostatic water (1mg/mL)
- Route
- Subcutaneous injection
- Frequency
- 4 days/week
Reconstitution Instructions
Using 1mL of bacteriostatic water results in a concentration of 1mg/mL. Always use sterile techniques.
Materials Needed
- 1mg Follistatin 344 1mg vial (lyophilized powder)
- 1mL bacteriostatic water (sterile, preserved with 0.9% benzyl alcohol)
- Sterile syringe and needle (18-22 gauge for drawing)
- Alcohol swabs
- Clean workspace
Step-by-Step Process
- Preparation: Allow the Follistatin 344 1mg vial and bacteriostatic water to reach room temperature. Wipe the rubber stoppers of both vials with an alcohol swab.
- Draw the Water: Using a sterile syringe, draw up exactly 1mL of bacteriostatic water. Expel any air bubbles.
- Inject into Vial: Insert the needle through the rubber stopper at a slight angle, aiming toward the glass wall. Slowly inject the water down the side of the vial to avoid foaming.
- Mix Gently: Remove the needle. Gently swirl or roll the vial between your hands. Do not shake vigorously.
- Check for Clarity: The solution should be clear once fully reconstituted. Do not use if cloudy.
Weekly Dosing Schedule
| Day | Units | Dose | Timing |
|---|---|---|---|
| Monday | 10 | 100mcg | Morning |
| Wednesday | 10 | 100mcg | Morning |
| Friday | 10 | 100mcg | Morning |
| Sunday | 10 | 100mcg | Morning |
Weekly Total: 40 units (400mcg) • Vial Duration: ~18 days (10 doses)
Quick Dosing Reference
| Units (Ticks) | Dose (mcg) | Dose (mg) |
|---|---|---|
| 5 | 50 | 0.05mg |
| 10 | 100 | 0.1mg |
| 20 | 200 | 0.2mg |
| 50 | 500 | 0.5mg |
| 100 | 1000 | 1mg |
Administration Guidelines
- Administer subcutaneously every other day
- 100mcg per dose is the standard research protocol
- Rotate injection sites between abdomen, thigh, and upper arm
- Myostatin inhibitor - studied for muscle growth and tissue remodeling
- Store reconstituted peptide refrigerated at 36-46F (2-8C)
- Use reconstituted solution within 30 days
- Never freeze reconstituted peptides
- Document administration times and observations
Storage Requirements
| State | Storage Conditions |
|---|---|
| Lyophilized (powder) | Room temperature or refrigerated, protect from light |
| Reconstituted | Refrigerated 36-46F (2-8C), use within 30 days |