Research & Educational Use Only. This protocol guide is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional before beginning any research protocol.
Upstream GnRH Stimulator / Fertility Research
Weekly Protocol Guide
Kisspeptin Protocol Guide
Kisspeptin is a neuropeptide that acts upstream of GnRH neurons in the hypothalamus. It is a critical regulator of reproductive hormone cascades and is researched for fertility applications, pubertal development, and as a diagnostic tool for hypothalamic function assessment.
Protocol Overview
- Compound
- Kisspeptin-10 (Metastin fragment 45-54)
- Category
- Reproductive / Endocrine Research Peptide
- Vial Size
- 5mg (5,000mcg)
- Reconstitution
- 2mL bacteriostatic water → 2.5mg/mL (2,500mcg/mL)
- Route
- Subcutaneous injection
- Frequency
- 2–3x per week
Reconstitution Instructions
Add 2mL of bacteriostatic water to a 5mg Kisspeptin-10 vial for a concentration of 2.5mg/mL (2,500mcg/mL). Always use sterile technique.
Materials Needed
- 5mg Kisspeptin-10 vial (lyophilized powder)
- 2mL bacteriostatic water (sterile, preserved with 0.9% benzyl alcohol)
- Sterile syringe and needle (18–22 gauge for drawing)
- Insulin syringe (29–31 gauge) for SubQ injection
- Alcohol swabs
- Clean workspace
Step-by-Step Process
- Allow the Kisspeptin-10 vial and bacteriostatic water to reach room temperature. Wipe rubber stoppers with an alcohol swab.
- Draw up exactly 2mL of bacteriostatic water using a sterile syringe. Expel air bubbles.
- Insert the needle through the rubber stopper at a slight angle and slowly inject the water down the side of the vial to avoid foaming.
- Remove the needle. Gently swirl or roll the vial. Do not shake vigorously.
- Solution should be clear. Final concentration: 2.5mg/mL (2,500mcg/mL). Store refrigerated.
Dosing Protocol
Kisspeptin-10 is dosed based on body weight (6–10 mcg/kg SubQ) or by flat dosing of 100–500mcg per injection. Start conservative and titrate based on hormonal response.
| Protocol | Dose | Volume (at 2.5mg/mL) | Frequency | Cycle |
|---|---|---|---|---|
| Conservative / Starter | 100mcg | 0.04mL (4 units) | 2–3x/week | 4–12 weeks |
| Standard SubQ | 200mcg | 0.08mL (8 units) | 2–3x/week | 4–12 weeks |
| Higher-Dose Research | 500mcg | 0.2mL (20 units) | 2–3x/week | 4–8 weeks |
Weight-based: 6–10 mcg/kg SubQ. A 75kg subject at 6 mcg/kg = 450mcg per dose. Vial lasts ~25 doses at 200mcg (5mg vial).
Quick Dosing Reference (at 2.5mg/mL — 5mg vial + 2mL BAC water)
| Units (Insulin Syringe) | Volume (mL) | Dose (mcg) | Dose (mg) |
|---|---|---|---|
| 4 | 0.04mL | 100mcg | 0.1mg |
| 8 | 0.08mL | 200mcg | 0.2mg |
| 12 | 0.12mL | 300mcg | 0.3mg |
| 20 | 0.2mL | 500mcg | 0.5mg |
Administration Guidelines
- Administer subcutaneously in the lower abdomen or thigh using a 29–31 gauge insulin syringe
- Rotate injection sites with each administration
- Dose 2–3x per week — typically 100–200mcg per injection for research protocols
- Timing: morning administration is common; some protocols dose pre-sleep for overnight LH pulse
- Store reconstituted peptide refrigerated at 36–46°F (2–8°C)
- Use reconstituted solution within 30 days
- Never freeze reconstituted peptides
- Cycle: 4–12 weeks on, with a break equal to cycle length before repeating
Storage Requirements
| State | Storage Conditions |
|---|---|
| Lyophilized (powder) | Room temperature or refrigerated, protect from light |
| Reconstituted (2.5mg/mL) | Refrigerated 36–46°F (2–8°C), use within 30 days |
Stacking Recommendations
Compatible Compounds
- Gonadorelin: Synergistic GnRH-axis stimulation (upstream + downstream)
- Enclomiphene: Selective estrogen receptor modulator for FSH/LH amplification
- HCG: Direct LH mimetic for testicular support alongside Kisspeptin
Popular Stacks
- Fertility Support: Kisspeptin-10 200mcg 2x/week + Gonadorelin 500mcg 3x/week
- PCT Protocol: Kisspeptin-10 200mcg 3x/week + Enclomiphene 12.5mg/day
- HPTA Maintenance: Kisspeptin-10 100mcg 2x/week + HCG 500 IU 2x/week