Thymosin Alpha-1 Protocol Guide
Thymosin Alpha-1 is a naturally occurring thymic peptide that modulates immune function. It enhances T-cell maturation, dendritic cell function, and antibody responses. It is researched for immune enhancement in chronic infections, immune deficiency, and as a vaccine adjuvant.
Protocol Overview
- Compound
- Thymosin Alpha-1 (Tα1; Zadaxin / Thymalfasin)
- Category
- Immune Modulation / Antiviral Research Peptide
- Vial Size
- 1.6mg (Zadaxin standard) or 10mg research vials
- Reconstitution
- 1.6mg vial + 1mL sterile water = 1.6 mg/mL
- Route
- Subcutaneous injection
- Frequency
- 2x per week (twice weekly)
- Cycle Length
- 8–16 weeks (immune support); ongoing for chronic conditions
Dosing Protocol
| Protocol | Dose | Frequency | Route | Duration |
|---|---|---|---|---|
| Conservative Start | 0.8 mg | 2x per week | Subcutaneous | 2–4 weeks, then assess |
| Standard (Zadaxin dose) | 1.6 mg | 2x per week (e.g. Mon & Thu) | Subcutaneous | 8–16 weeks |
| Higher Immune Support | 1.6 mg | 3x per week | Subcutaneous | 8–12 weeks |
CRITICAL DOSING NOTE: The standard dose is 1.6 mg (1,600 mcg) — NOT 25–100 mcg. Thymosin Alpha-1 is dosed in milligrams, not micrograms like many other peptides. The Zadaxin clinical dose of 1.6 mg twice weekly is based on FDA-approved human data. Underdosing at 25 mcg would be approximately 64x too low to achieve immune modulation.
Reconstitution Instructions
For a 1.6mg vial: add 1mL bacteriostatic water for a concentration of 1.6 mg/mL. For a 10mg research vial: add 6.25mL for 1.6 mg/mL. Always use sterile techniques.
Materials Needed
- Thymosin Alpha-1 vial (1.6mg Zadaxin-standard or 10mg research vial)
- Bacteriostatic water (1mL per 1.6mg vial)
- Sterile syringe and needle (18-22 gauge for drawing)
- Insulin syringes (29-31G) for dosing
- Alcohol swabs
- Clean workspace
Step-by-Step Process
- Allow vial and bacteriostatic water to reach room temperature. Wipe rubber stopper with alcohol swab.
- Draw up 1mL of bacteriostatic water. Expel air bubbles.
- Inject slowly down the vial wall to avoid foaming.
- Gently swirl or roll. Do not shake vigorously.
- Solution should be clear. Do not use if cloudy.
- Concentration (1.6mg + 1mL): 1.6 mg/mL = 1,600 mcg/mL
Weekly Dosing Schedule (Standard 1.6 mg Twice Weekly)
| Day | Units (100-unit syringe) | Dose | Timing |
|---|---|---|---|
| Monday | 100 units (1 full syringe at 1.6mg/mL) | 1.6 mg (1,600 mcg) | Morning |
| Thursday | 100 units (1 full syringe at 1.6mg/mL) | 1.6 mg (1,600 mcg) | Morning |
Weekly Total: 3.2 mg • Vial Duration: 1 vial (1.6mg) per injection
For 10mg research vials reconstituted in 6.25mL (1.6mg/mL): draw 1mL per dose. Vial provides approximately 6 full doses.
Quick Dosing Reference (1.6 mg/mL concentration)
| Units (Ticks) | Dose (mcg) | Dose (mg) |
|---|---|---|
| 25 | 400 mcg | 0.4 mg |
| 50 | 800 mcg | 0.8 mg (conservative dose) |
| 100 | 1,600 mcg | 1.6 mg (standard Zadaxin dose) |
Administration Guidelines
- Administer subcutaneously in the abdomen or thigh
- Rotate injection sites with each administration
- Standard research dose: 1.6 mg SubQ twice weekly (Mon and Thu is a common schedule)
- Conservative start: 0.8 mg twice weekly for the first 2 weeks
- Higher immune support: 1.6 mg three times per week (for acute immune challenges)
- Cycle length: 8–16 weeks for chronic immune support; ongoing use for long-term maintenance
- Store reconstituted peptide refrigerated at 36–46°F (2–8°C)
- Use reconstituted solution within 30 days
- Never freeze reconstituted peptides
- Document administration times and observations
Compatible Compounds
Common Stacks
- BPC-157: Tissue repair and systemic anti-inflammatory support
- LL-37: Complementary antimicrobial and innate immune activation
- TB-500 (Thymosin Beta-4): Tissue regeneration and immune modulation
- LDN (Low Dose Naltrexone): Commonly paired in chronic immune conditions
Research Applications
- Chronic infections (hepatitis B/C, HIV-associated)
- Immune deficiency and T-cell enhancement
- Vaccine adjuvant (enhances immune response)
- Cancer immunotherapy support (preclinical)
- Autoimmune conditions (immune normalization)
Storage Requirements
| State | Storage Conditions |
|---|---|
| Lyophilized (powder) | Room temperature or refrigerated, protect from light |
| Reconstituted | Refrigerated 36–46°F (2–8°C), use within 30 days |