Research & Educational Use Only. This protocol guide is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional before beginning any research protocol.
Mitochondrial-Derived Longevity Peptide
Weekly Protocol Guide
Humanin Protocol Guide
Humanin is a mitochondrial-derived peptide encoded in the 16S rRNA region, studied for cytoprotective and longevity-promoting properties. Research suggests it may protect against oxidative stress, reduce apoptosis, and improve metabolic function. It interacts with IGFBP-3 and BAX pathways.
Protocol Overview
- Compound
- Humanin (Mitochondrial Open Reading Frame Cytoprotective Peptide)
- Category
- Mitochondrial-Derived Longevity Peptide
- Vial Size
- 5mg (5,000mcg)
- Reconstitution
- 2mL bacteriostatic water (2.5mg/mL)
- Route
- Subcutaneous injection
- Frequency
- 7 days/week
Reconstitution Instructions
Using 2mL of bacteriostatic water results in a concentration of 2.5mg/mL (2500mcg/mL). Always use sterile techniques.
Materials Needed
- 5mg Humanin 5mg vial (lyophilized powder)
- 2mL bacteriostatic water (sterile, preserved with 0.9% benzyl alcohol)
- Sterile syringe and needle (18-22 gauge for drawing)
- Alcohol swabs
- Clean workspace
Step-by-Step Process
- Preparation: Allow the Humanin 5mg vial and bacteriostatic water to reach room temperature. Wipe the rubber stoppers of both vials with an alcohol swab.
- Draw the Water: Using a sterile syringe, draw up exactly 2mL of bacteriostatic water. Expel any air bubbles.
- Inject into Vial: Insert the needle through the rubber stopper at a slight angle, aiming toward the glass wall. Slowly inject the water down the side of the vial to avoid foaming.
- Mix Gently: Remove the needle. Gently swirl or roll the vial between your hands. Do not shake vigorously.
- Check for Clarity: The solution should be clear once fully reconstituted. Do not use if cloudy.
Weekly Dosing Schedule
| Day | Units | Dose | Timing |
|---|---|---|---|
| Monday | 4 | 100mcg | Morning |
| Tuesday | 4 | 100mcg | Morning |
| Wednesday | 4 | 100mcg | Morning |
| Thursday | 4 | 100mcg | Morning |
| Friday | 4 | 100mcg | Morning |
| Saturday | 4 | 100mcg | Morning |
| Sunday | 4 | 100mcg | Morning |
Weekly Total: 28 units (700mcg) • Vial Duration: ~71 days
Quick Dosing Reference
| Units (Ticks) | Dose (mcg) | Dose (mg) |
|---|---|---|
| 2 | 50 | 0.05mg |
| 4 | 100 | 0.1mg |
| 8 | 200 | 0.2mg |
| 10 | 250 | 0.25mg |
| 20 | 500 | 0.5mg |
Administration Guidelines
- Administer subcutaneously daily in the morning
- Rotate injection sites between abdomen, thigh, and upper arm
- Store reconstituted peptide refrigerated at 36-46F (2-8C)
- Use reconstituted solution within 30 days
- Never freeze reconstituted peptides
- Document administration times and observations
Storage Requirements
| State | Storage Conditions |
|---|---|
| Lyophilized (powder) | Room temperature or refrigerated, protect from light |
| Reconstituted | Refrigerated 36-46F (2-8C), use within 30 days |