Research & Educational Use Only. Not medical advice. Consult a healthcare professional.
Longevity / Cellular EnergyEvidence Grade: B+ (Human + Preclinical)
NAD+ Protocol Guide
NAD+ (Nicotinamide Adenine Dinucleotide) is a coenzyme present in every living cell, essential for energy metabolism, DNA repair, sirtuin activation, and cellular signaling. NAD+ levels decline with age, making supplementation a key focus of longevity research. This protocol covers subcutaneous injection dosing at 100-500mg daily, reconstitution from a 1000mg vial, IV infusion protocols, expected timeline for energy and cognitive benefits, and stacking with other longevity compounds.
Protocol Overview
- Compound
- NAD+ (Nicotinamide Adenine Dinucleotide)
- Category
- Coenzyme / Longevity
- Mechanism
- Essential coenzyme for mitochondrial energy production (Complex I), sirtuin activation (SIRT1-7), PARP-mediated DNA repair, and CD38 signaling
- MW
- 663.43 Da
- Half-Life
- ~2-4 hours (serum)
- Vial
- 1000mg lyophilized
- Route
- Subcutaneous, IV infusion, or oral (NMN/NR precursors)
- Frequency
- Daily (loading) / 2-3x weekly (maintenance)
- Cycle
- 4-12+ weeks
Dosing
| Protocol | Phase | Dose | Frequency | Route |
|---|---|---|---|---|
| SubQ Loading | Week 1-4 | 200-500 mg | Daily | Subcutaneous |
| SubQ Maintenance | Week 5+ | 100-200 mg | 2-3x weekly | Subcutaneous |
| IV Infusion | As scheduled | 250-750 mg | 1-2x weekly | IV drip (2-4 hours) |
| Oral (NMN) | Ongoing | 500-1000 mg NMN | Daily | Oral |
Reconstitute 1000mg with 10mL BAC water = 100mg/mL. 200mg = 2mL = split into 2x 1mL injections. SubQ injection stings - inject slowly.
Timeline
Day 1-7
Immediate energy and mental clarity improvements (especially IV). Improved sleep onset. Mild flushing.
Week 2-4
Sustained energy improvements. Reduced brain fog. Better exercise recovery. Cellular NAD+ levels rising.
Week 5-8
Cumulative longevity benefits. Improved metabolic markers. Skin quality improvements. Enhanced mitochondrial function.
Week 9-12+
Sustained cellular optimization. Ongoing sirtuin activation. Consider long-term maintenance protocol.
Side Effects & Stacking
Side Effects
- Injection site stinging/burning (common)
- Nausea at higher doses
- Flushing and warmth
- Chest tightness during IV (rate-dependent)
- Headache (transient)
Longevity Stack
- GHK-Cu: Anti-aging peptide synergy
- Epithalon: Telomere support
- MOTS-C: Mitochondrial peptide
- Glutathione: Master antioxidant
- Resveratrol: Sirtuin activator (oral)
Blood Work
| Panel | Markers | Timing |
|---|---|---|
| NAD+ Levels | Intracellular NAD+ (specialized lab) | Baseline, Week 4 |
| Liver | ALT, AST | Baseline, Week 4 |
| Metabolic | Fasting glucose, insulin | Baseline, Week 8 |
| Basic | CBC, CMP | Baseline, Week 8 |
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