Research & Educational Use Only. This protocol guide is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional before beginning any research protocol.
PEGylated Mechano Growth Factor
Weekly Protocol Guide
PEG-MGF Protocol Guide
PEG-MGF is a PEGylated form of Mechano Growth Factor, a splice variant of IGF-1. The PEG modification extends its half-life significantly. It is studied for satellite cell activation and muscle repair post-exercise. Administered subcutaneously 3 times per week after exercise.
Protocol Overview
- Compound
- PEG-MGF (PEG-MGF (PEGylated Mechano Growth Factor))
- Category
- PEGylated Mechano Growth Factor
- Vial Size
- 2mg (2,000mcg)
- Reconstitution
- 2mL bacteriostatic water (1mg/mL)
- Route
- Subcutaneous injection
- Frequency
- 3 days/week
Reconstitution Instructions
Using 2mL of bacteriostatic water results in a concentration of 1mg/mL. Always use sterile techniques.
Materials Needed
- 2mg PEG-MGF 2mg vial (lyophilized powder)
- 2mL bacteriostatic water (sterile, preserved with 0.9% benzyl alcohol)
- Sterile syringe and needle (18-22 gauge for drawing)
- Alcohol swabs
- Clean workspace
Step-by-Step Process
- Preparation: Allow the PEG-MGF 2mg vial and bacteriostatic water to reach room temperature. Wipe the rubber stoppers of both vials with an alcohol swab.
- Draw the Water: Using a sterile syringe, draw up exactly 2mL of bacteriostatic water. Expel any air bubbles.
- Inject into Vial: Insert the needle through the rubber stopper at a slight angle, aiming toward the glass wall. Slowly inject the water down the side of the vial to avoid foaming.
- Mix Gently: Remove the needle. Gently swirl or roll the vial between your hands. Do not shake vigorously.
- Check for Clarity: The solution should be clear once fully reconstituted. Do not use if cloudy.
Weekly Dosing Schedule
| Day | Units | Dose | Timing |
|---|---|---|---|
| Monday | 20 | 200mcg | Post-exercise |
| Wednesday | 20 | 200mcg | Post-exercise |
| Friday | 20 | 200mcg | Post-exercise |
Weekly Total: 60 units (600mcg) • Vial Duration: ~10 doses (~3.3 weeks)
Quick Dosing Reference
| Units (Ticks) | Dose (mcg) | Dose (mg) |
|---|---|---|
| 5 | 50 | 0.05mg |
| 10 | 100 | 0.1mg |
| 20 | 200 | 0.2mg |
| 50 | 500 | 0.5mg |
| 100 | 1000 | 1mg |
Administration Guidelines
- Administer subcutaneously post-exercise, 3 times per week
- 200mcg per dose is the standard research protocol
- Inject into or near the muscle group trained for localized effect
- Rotate injection sites between trained muscle groups
- Satellite cell activator - best used on training days after workout
- Store reconstituted peptide refrigerated at 36-46F (2-8C)
- Use reconstituted solution within 30 days
- Never freeze reconstituted peptides
- Document administration times and observations
Storage Requirements
| State | Storage Conditions |
|---|---|
| Lyophilized (powder) | Room temperature or refrigerated, protect from light |
| Reconstituted | Refrigerated 36-46F (2-8C), use within 30 days |